熱甩尾儀根據(jù)D"amour/Smith原理和方法測量大鼠、小鼠尾巴部受紅外熱刺激時的痛覺閾值。

實驗步驟及方法

· 該儀器使用可調(diào)強度的紅外光源紅外光通過一拋物鏡面反射聚焦在實驗動物的尾巴上；

· 操作人員將實驗動物置于儀器上把動物的尾巴放在紅外光源處接受熱輻射刺激；

· 當動物感覺到疼痛時尾巴會左右甩動或者輕敲臺面內(nèi)置傳感器會立刻檢測到尾巴的活動停止計時和關閉光源；

· 儀器自動記錄反應時間和光源強；

· 數(shù)據(jù)可通過U盤或USB數(shù)據(jù)線導出到電腦；

· 該型號的設備在世界上使用和驗證超過3000次積累了數(shù)十年的經(jīng)驗；

型號37560

主要特點

· 尾部輕拍可自動記錄或手動評分；
· 自動化程度高避免人為因素引起的誤差；
· 觸摸屏控制操控方便顯示直觀；
· 可獨立工作也可連接電腦使用；
· 測試平臺平整表面無突出和遮擋部件；
· 可選配紅外熱輻射校準儀用于校準紅外光源；
· 多種單位可轉換；

· 該儀器適合大鼠和小鼠的測試；

· 有大鼠、小鼠兩種不同的固定器可供選擇；

正在進行大鼠甩尾測試

儀器的操作
· 主機內(nèi)包括紅外光源、傳感器、微控制器；
· 自動檢測尾巴的活動情況自動計時延遲時間會被自動記錄；
· 可選配傾斜的老鼠固定器將老鼠尾巴保持在 45 度向上的位置。

可選配的小鼠固定器

控制面板

· 數(shù)據(jù)格式通用化可使用其他軟件進行統(tǒng)計；
· 帶儲存功能用于實驗數(shù)據(jù)的保存；

· 可聯(lián)網(wǎng)通過遠程網(wǎng)絡對實驗方案進行編輯；

校準輻射計
· 用來對輻射強度進行校準；校準輻射計貨號我37300；
· 確保兩個或多個裝置提供相同強度的熱傷害性刺激（輻射單位mW/cm2）
· 測量紅外光的能量的值（1s持續(xù)時間內(nèi)的1mW對應1mJ）

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方法學文獻

F.E. D’mour & D.L. Smith"A Method for Determining Loss of Pain Sensation." J. Pharmacol. Exp. Therap. 7274-79, 1941.